DUX4 antibody (C-Term)

Catalog No. ABIN6655475

Product Details anti-DUX4 Antibody

Target: DUX4 (Double Homeobox 4 (DUX4))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DUX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Purification: Anti-DUX4 Antibody was purified by Protein G chromatography. This monoclonal antibody is specific for human DUX4 protein. A BLAST analysis was used to suggest cross-reactivity with DUX4 from human based on 100% homology with the immunizing sequence. It does not cross react with DUX4c. Cross-reactivity with DUX4 from other sources has not been determined. Transcription Factor research.

Immunogen:

Immunogen: DUX4 Antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the C-terminal region of human DUX4 protein.

Immunogen Type: Peptide

Clone: P2B1

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-DUX4 Antibody is suitable for use in WB, IF, and IHC. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for DUX4

Target: DUX4 (Double Homeobox 4 (DUX4))

Alternative Name: DUX4 (DUX4 Products)

Synonyms: DUX4 antibody, DUX4L antibody, double homeobox, 4 antibody, double homeobox 4 antibody, DUX4 antibody

Background:

Synonyms: mouse anti-DUX4 Antibody, double homeobox 4, DUX4, Double homeobox protein 4-like protein, DUX4L2, DUX4L3, DUX4L5, DUX4L6, DUX4L7

Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1. Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function. In FSHD individuals, the expression of the full-length DUX4 transcipt is not completely suppressed in skeletal muscle and possibly other differentiated tissues.

Gene Name: DUX2

Gene ID: 22947

NCBI Accession: NP_149418

UniProt: F5GZ66