CD27 antibody (FITC)
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- Target See all CD27 Antibodies
- CD27 (CD27 Molecule (CD27))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD27 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Cross-Reactivity (Details)
- Cross reactivity with CD27 from other sources has not been tested
- Purification
- Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey. . Anti-CD27 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
- Immunogen
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Immunogen: Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
- Clone
- O323
- Isotype
- IgG1
- Labeling Ratio
- 4-6
- Top Product
- Discover our top product CD27 Primary Antibody
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- Application Notes
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Application Note: Anti-CD27 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
0.09 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).
: "CD27: marker and mediator of T-cell activation?" in: Immunology today, Vol. 15, Issue 7, pp. 307-11, (1994) (PubMed).
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Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).
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- Target
- CD27 (CD27 Molecule (CD27))
- Alternative Name
- CD27 (CD27 Products)
- Synonyms
- TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
- Background
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Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Background: CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.
Gene Name: CD27
- Gene ID
- 939
- NCBI Accession
- NP_001233
- UniProt
- P26842
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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