Parkin antibody (pSer378)

Catalog No. ABIN6655557

Supplier Product No.: 609-401-e07

This Rabbit Polyclonal antibody specifically detects Parkin in WB. It exhibits reactivity toward Human.

Quick Overview for Parkin antibody (pSer378) (ABIN6655557)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Parkin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Parkin Antibody

Binding Specificity: pSer378

Supplier Product No.: 609-401-e07

Supplier: Rockland

Purpose: Parkin phospho S378 Antibody

Cross-Reactivity (Details): Anti-Parkin pS378 antibody is directed against human Parkin protein.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Parkin pS378 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Parkin pS378 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 52 kDa in size corresponding to parkin protein phosphorylated at Ser378 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: Parkin

Background:

Synonyms: rabbit anti-Parkin pS378 Antibody, E3 ubiquitin-protein ligase parkin, Parkinson juvenile disease protein 2, Parkinson disease protein 2

Background: Parkin pS378 Antibody detects Parkin pS378 protein. Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra. Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity. Anti-Parkin pS378 Antibody is ideal for investigators involved in Neuroscience, Signal Transduction, and Apoptosis research.

Gene Name: PARK2

Gene ID: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway