PAPOLA antibody (C-Term)

Catalog No. ABIN6655568

Product Details anti-PAPOLA Antibody

Target: PAPOLA (Poly(A) Polymerase alpha (PAPOLA))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPOLA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with PAPOLA from other species not tested.

Purification: Anti-PAPOLA Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Immunogen:

Immunogen: Anti-PAPOLA Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human PAPOLA containing an amino acid sequence from the C-terminus of the protein.

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-PAPOLA Antibody is suitable for ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 83 kDa in the appropriate cell lysate or extract.

ELISA Dilution: 1:100 - 1:500

Western Blot Dilution: 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for PAPOLA

Target: PAPOLA (Poly(A) Polymerase alpha (PAPOLA))

Alternative Name: PAPOLA (PAPOLA Products)

Background:

Synonyms: Poly(A) polymerase alpha, PAP-alpha, Polynucleotide adenylyltransferase alpha, PAP

Background: PAPOLA is an RNA polymerase that generates the poly(A) tail of RNA's by adding AMP monomers to the 3'-end. PAPOLA may also be required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. Anti-PAPOLA Antibody is ideal for research in Gene Expression, Transcription and Translation.

Gene Name: PAPOLA

Gene ID: 10914

NCBI Accession: NP_001238935

UniProt: P51003