Bax Inhibitor 1 antibody

Catalog No. ABIN6655585

Supplier Product No.: 200-301-h60

The Mouse Monoclonal anti-Bax Inhibitor 1 antibody has been validated for WB and IHC. It is suitable to detect Bax Inhibitor 1 in samples from Human.

Quick Overview for Bax Inhibitor 1 antibody (ABIN6655585)

Target: Bax Inhibitor 1 (TMBIM6) (Transmembrane BAX Inhibitor Motif Containing 6 (TMBIM6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bax Inhibitor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 20F430

Product Details anti-Bax Inhibitor 1 Antibody

Supplier Product No.: 200-301-h60

Supplier: Rockland

Purpose: BI1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Bi-1 from human, mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Bi-1 Antibody was purified by Protein G chromatography.

Immunogen: Bi-1 Antibody was produced in mice prepared by repeated immunizations of a mixture of three synthetic peptides corresponding to the N-terminus, internal and C-terminus of human Bi-1 protein.

Isotype: IgG2b kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 1:500-1:1000

Comment:

Anti-Bi-1 antibody is tested for use in WB and IHC-P. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % Gelatin

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Bax Inhibitor 1

Target: Bax Inhibitor 1 (TMBIM6) (Transmembrane BAX Inhibitor Motif Containing 6 (TMBIM6))

Alternative Name: Bi-1

Background:

Synonyms: BI1, TEGT, Bax inhibitor 1, BI-1, Testis-enhanced gene transcript protein,Transmembrane BAX inhibitor motif-containing protein 6

Background: Anti-Bi-1 antibody detects human Bi-1. BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress-causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, it is thought to involve regulation of Ca2+ handling by the ER. Anti-Bi-1 antibody is ideal for investigators involved in apoptosis research.

Gene Name: TMBIM6

Gene ID: 7009

NCBI Accession: NP_001092046

UniProt: P55061

Pathways: Response to Water Deprivation, ER-Nucleus Signaling