KDM1B antibody (Internal Region, N-Term)

Catalog No. ABIN6655594

Supplier Product No.: 100-401-w43

The Rabbit Polyclonal anti-KDM1B antibody has been validated for ELISA and WB. It is suitable to detect KDM1B in samples from Human.

Quick Overview for KDM1B antibody (Internal Region, N-Term) (ABIN6655594)

Target: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM1B antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-KDM1B Antibody

Binding Specificity: Internal Region, N-Term

Supplier Product No.: 100-401-w43

Supplier: Rockland

Purpose: KDM1B Antibody

Cross-Reactivity (Details): Cross reactivity with KDM1B from other species not tested.

Purification: Anti-KDM1B Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-KDM1B Antibody was produced in rabbits by repeated immunizations with two different synthetic peptides containing a sequence from the N-terminus and internal region of the human KDM1B protein.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:500

Comment:

Anti-KDM1B Antibody has been tested by ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KDM1B

Target: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Alternative Name: KDM1B

Background:

Synonyms: Lysine-specific histone demethylase 1B, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, AOF1, C6orf193, LSD2

Background: KDM1B is a histone demethylase that demethylates lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1B is able to demethylate both mono- and di-methylated lysine 4 of histone H3, but has no effect on tri-methylated lysine 4, mono-, di- or tri-methylated lysine 9, mono-, di- or tri-methylated lysine 27, mono-, di- or tri-methylated lysine 36 of histone H3, or on mono-, di- or tri-methylated lysine 20 of histone H4. KDM1B is also required for de novo DNA methylation of a subset of imprinted genes during oogenesis. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Anti-KDM1B is ideal for research in Epigenetics and Gene Expression.

Gene Name: KDM1B

Gene ID: 221656

NCBI Accession: NP_694587

UniProt: Q8NB78

Pathways: Warburg Effect