Tumor Protein p73 antibody

Catalog No. ABIN6655655

Supplier Product No.: 200-301-i00

The Mouse Monoclonal anti-Tumor Protein p73 antibody (Clone 5B429) (ABIN6655655) specifically detects Tumor Protein p73 in WB, IHC, IF, IP, FACS and ChIP. The antibody is reactive with Human samples.

Quick Overview for Tumor Protein p73 antibody (ABIN6655655)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)

Clone: 5B429

Product Details anti-Tumor Protein p73 Antibody

Supplier Product No.: 200-301-i00

Supplier: Rockland

Purpose: p73 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-p73 from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-p73 Antibody was purified by Protein G chromatography.

Immunogen: p73 Antibody was produced in mice prepared by repeated immunizations with full-length human p73 protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5-15 μg/mL

Western_Blot_Dilution: 2 μg/mL

Comment:

Anti-p73 Antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 70kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: p73

Background:

Synonyms: P73, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: Anti-p73 detects human p73. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa. Anti-p73 antibody is ideal for investigators involved in NFkappaB, kinase and phosphatse research.

Gene Name: TP73

Gene ID: 7161

NCBI Accession: NP_001119712

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus