Tumor Protein p73 antibody

Catalog No. ABIN6655656

Supplier Product No.: 200-301-i01

This Mouse Monoclonal antibody specifically detects Tumor Protein p73 in WB, IHC, IF, IP and ChIP. It exhibits reactivity toward Human.

Quick Overview for Tumor Protein p73 antibody (ABIN6655656)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 5B1288

Product Details anti-Tumor Protein p73 Antibody

Supplier Product No.: 200-301-i01

Supplier: Rockland

Purpose: p73 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-p73 from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-p73 Antibody was purified by Protein G chromatography.

Immunogen: p73 Antibody was produced in mice prepared by repeated immunizations with full-length human p73 protein.

Isotype: IgG1 kappa

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1-3 μg/mL

Comment:

Anti-p73 Antibody is tested for use in WB, ChIP, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 70kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: p73

Background:

Synonyms: P73, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: Anti-p73 antibody detects human p73. p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63 % amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer. Anti-p73 antibody is ideal for investigators involved in cell cycle protein research.

Gene Name: TP73

Gene ID: 7161

NCBI Accession: NP_001119712

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus