CRYAB antibody

Catalog No. ABIN6655665

Supplier Product No.: 200-301-e85

This Mouse Monoclonal antibody specifically detects CRYAB in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for CRYAB antibody (ABIN6655665)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 1A7-D5

Product Details anti-CRYAB Antibody

Supplier Product No.: 200-301-e85

Supplier: Rockland

Purpose: Alpha B Crystallin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with alpha B crystallin from Human and bovine based on 100 % homology with the immunizing sequence.

Purification: Anti-Alpha B Crystallin Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Alpha B Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha B Crystallin protein.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Comment:

Anti-Alpha B Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa (Predicted mol. weight is ~21kDa) corresponding to αB-crystallin. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: Alpha B Crystallin

Background:

Synonyms: AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases. Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions.

Gene Name: CRYAB

Gene ID: 1410

NCBI Accession: NP_001876

UniProt: P02511