TNFRSF11A antibody

Catalog No. ABIN6655792

Supplier Product No.: 200-301-i06

The Mouse Monoclonal anti-TNFRSF11A antibody is suitable to detect TNFRSF11A in samples from Human. It has been validated for WB, IHC and FACS.

Quick Overview for TNFRSF11A antibody (ABIN6655792)

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF11A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 9A725

Product Details anti-TNFRSF11A Antibody

Supplier Product No.: 200-301-i06

Supplier: Rockland

Purpose: RANK Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-RANK from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-RANK Antibody was purified by Protein G chromatography.

Immunogen: RANK Antibody was produced in mice prepared by repeated immunizations with a fusion protein containing internal amino acids from the human RANK protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Flow_Cytometry_Dilution: 2-4 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-RANK Antibody is tested for use in WB, Flow, Flow-CS, IHC, and IHC-P. Expect a band approximately 90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TNFRSF11A

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: RANK

Background:

Synonyms: RANK, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, ODFR, Receptor activator of NF-KB, CD265

Background: Anti-RANK Antibody detects human Rank. Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. RANK, or Receptor activator of NF-kB, TNF11aR, ODFR, CD265, is a member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2. Anti-RANK Antibody is ideal for investigators involved in apoptosis and NFkappaB research.

Gene Name: TNFRSF11A

Gene ID: 8792

NCBI Accession: NP_003830

UniProt: Q9Y6Q6

Pathways: NF-kappaB Signaling