DNMT1 antibody

Catalog No. ABIN6655855

Supplier Product No.: 200-301-h69

The Mouse Monoclonal anti-DNMT1 antibody has been validated for WB, IHC, ELISA, IP, IF, ChIP and FACS. It is suitable to detect DNMT1 in samples from Human.

Quick Overview for DNMT1 antibody (ABIN6655855)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS)

Clone: 60B1220-1

Product Details anti-DNMT1 Antibody

Supplier Product No.: 200-301-h69

Supplier: Rockland

Purpose: DNMT1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Dnmt1 from human, mouse and Zebrafish based on 100 % homology with the immunizing sequence.

Purification: Anti-Dnmt1 Antibody was purified by Protein G chromatography.

Immunogen: Dnmt1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with Dnmt1 human protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

ELISA_Dilution: 0.1-0.5 μg/mL

Immunohistochemistry_Dilution: 1-2 μg/mL

Flow_Cytometry_Dilution: 1-5 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Comment:

Anti-DNMT1 antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, and IP. Expect a band approximately 183 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: Dnmt1

Background:

Synonyms: AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Background: Anti-Dnmt1 antibody detects human Dnmt1. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Anti-Dnmt1 antibody is ideal for investigators involved in cancer, cell cycle protein research and epigenetics.

Gene Name: DNMT1

Gene ID: 1786

NCBI Accession: NP_001124295

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection