TJP1 antibody

Catalog No. ABIN6655950

Supplier Product No.: 600-401-gu7s

This Rabbit Polyclonal antibody specifically detects TJP1 in WB, IHC, ELISA, IF, FACS, FM and MA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for TJP1 antibody (ABIN6655950)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Product Details anti-TJP1 Antibody

Supplier Product No.: 600-401-gu7s

Supplier: Rockland

Purpose: ZO-1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ZO-1.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 10,000-1:50,000

Immunohistochemistry_Dilution: 1:100 - 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-TJP1 antibody (ABIN6655950)

Singh, Zabbarova, Ikeda, Maranchie, Chermansky, Foley, Hitchens, Yoshimura, Kanai, Kaufman, Tyagi: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: ZO-1

Background:

Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

Gene Name: TJP1

Gene ID: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization