CRYAB antibody

Catalog No. ABIN6656044

Supplier Product No.: 100-401-f18

The Rabbit Polyclonal anti-CRYAB antibody has been validated for WB and IHC. It is suitable to detect CRYAB in samples from Human.

Quick Overview for CRYAB antibody (ABIN6656044)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CRYAB Antibody

Supplier Product No.: 100-401-f18

Supplier: Rockland

Purpose: Alpha B Crystallin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Crystallin Alpha B from Human, Mouse, Rat, Cow, and Chicken based on 100 % homology with the immunizing sequence.

Purification: Anti-Crystallin Alpha B Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: Crystallin Alpha B Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to human alpha B crystallin.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:5000-10000

Comment:

Anti-Crystallin Alpha B Antibody is suitable for use in WB, IHC and IF microscopy. Expect a band approximately ~22kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: Crystallin Alpha B

Background:

Synonyms: AACRYA, Alpha crystallin B chain, CRYA2, CRYAB, CTPP2, HspB5, NY REN 27 antigen, Rosenthal fiber component, Renal carcinoma antigen NY-REN-27, Heat shock protein beta-5

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases. Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions.

Gene Name: CRYAB

Gene ID: 1410

NCBI Accession: NP_001876

UniProt: P02511