ENO2/NSE antibody

Catalog No. ABIN6656045

Supplier Product No.: 100-401-e00

This anti-ENO2/NSE antibody is a Rabbit Polyclonal antibody detecting ENO2/NSE in WB, IHC, IF, MA and FM. Suitable for Human.

Quick Overview for ENO2/NSE antibody (ABIN6656045)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Product Details anti-ENO2/NSE Antibody

Supplier Product No.: 100-401-e00

Supplier: Rockland

Purpose: Neuron specific enolase (NSE) Antibody

Cross-Reactivity (Details): This antibody is a monospecific neat serum.

Purification: Anti-NSE antibody directed against human neuron specific enolase protein.

Immunogen: Anti-NSE Antibody was produced by repeated immunizations with recombinant human NSE expressed in E. coli.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2000

Other: User Optimized

Comment:

Anti-Neuron specific enolase (Rabbit) antibody has been tested by Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in size corresponding to NSE protein by western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron specific enolase (NSE)

Background:

Synonyms: Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, NSE

Background: NSE antibody detects NSE protein. Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. Anti-neuron specific enolase (NSE) antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: ENO2

Gene ID: 2026

UniProt: P09104