HSF1 antibody

Catalog No. ABIN6656048

Supplier Product No.: 200-501-f50

This anti-HSF1 antibody is a Rat Monoclonal antibody detecting HSF1 in WB, ELISA, IHC, IP, GS, FACS and FM. Suitable for Human.

Quick Overview for HSF1 antibody (ABIN6656048)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Gel Shift (GS), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 10H8

Product Details anti-HSF1 Antibody

Supplier Product No.: 200-501-f50

Supplier: Rockland

Purpose: HSF1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with HSF1 from Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, and Rabbit based on 100 % homology with the immunizing sequence.

Purification: Anti-HSF1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: HSF1 Antibody was produced in rat by repeated immunizations with purified recombinant HSF1 protein.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

IF_Microscopy_Dilution: 10 μg/mL

Gel_Shift_Dilution: User Optimized

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-HSF1 Antibody is tested for WB, IP, IF microscopy, and IHC. Expect a band approximately ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Synonyms: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization. There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form. Various experiments have suggested that HFS2 may have roles in differentiation and development.

Gene Name: HSF1

Gene ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613