HSPE1 antibody (C-Term)

Catalog No. ABIN6656049

Product Details anti-HSPE1 Antibody

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis

Purification: Anti-Cpn10 Antibody was purified by Protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with Cpn 10 from Human, Mouse, Rat, Bovine, Canine, Guinea pig, Pig, Rabbit, Sheep, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Cpn10 from other sources has not been determined. Heat Shock research.

Immunogen:

Immunogen: Cpn10 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the c-terminal region of human Cpn10 synthetic peptide.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Cpn10 Antibody is suitable for use in WB, ELISA, IHC, IF, and IP. Expect a band approximately ~10 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:200

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for HSPE1

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Alternative Name: Cpn10 (HSPE1 Products)

Synonyms: CPN10 antibody, EPF antibody, GROES antibody, HSP10 antibody, groS antibody, Cpn10 antibody, cpn10 antibody, zgc:86747 antibody, hspe1 antibody, MGC89647 antibody, HSPE1 antibody, MITOCHONDRIAL CHAPERONIN 10 antibody, T15D22.2 antibody, T15D22_2 antibody, chaperonin 10 antibody, Dmel\\CG11267 antibody, Hsp10 antibody, anon-WO0118547.175 antibody, hsp10 antibody, crpB antibody, chpS antibody, 10kDa antibody, mt-cpn10 antibody, heat shock protein family E (Hsp10) member 1 antibody, molecular chaperone GroES antibody, chaperonin 10 kDa antibody, chaperonin, 10 kDa antibody, chaperonin-10 kDa antibody, heat shock protein family E member 1 antibody, heat shock 10 protein 1 antibody, heat shock protein family E (Hsp10) member 1 L homeolog antibody, heat shock 10kDa protein 1 (chaperonin 10) pseudogene antibody, 10 kd chaperonin antibody, chaperonin 10 antibody, Hsp10p antibody, co-chaperonin 10, mitochondrial antibody, CG11267 gene product from transcript CG11267-RA antibody, co-chaperonin GroES antibody, mitochondrial heat shock protein Hsp10 (predicted) antibody, chaperonin GroS antibody, Hsp10 10 kDa chaperonin GROES antibody, mitochondrial heat shock protein Hsp10 antibody, heat shock protein 10 antibody, heat shock protein 1 (chaperonin 10) antibody, heat shock 10kDa protein 1 (chaperonin 10) antibody, HSPE1 antibody, groES antibody, groES2 antibody, TP01_0190 antibody, Cag_1167 antibody, Bm1_56470 antibody, Hspe1 antibody, hspe1 antibody, hspe1.L antibody, LOC452179 antibody, Cpn10 antibody, CPN10 antibody, HSP10 antibody, CG11267 antibody, hsp10 antibody, groS antibody

Background:

Synonyms: 10 kDa Chaperonin, Chaperonin 10, Cpn 10, EPF, GROES, Heat Shock 10kD protein1, Hsp10, HSPE1, Early-pregnancy factor

Background: Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process. Cpn10 has been located in human platelets, but is also present in human maternal serum. It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy.

Gene Name: HSPE1

Gene ID: 3336

NCBI Accession: NP_002148

UniProt: P61604

Pathways: Positive Regulation of Endopeptidase Activity