HSP70 1A antibody (AA 436-503)

Catalog No. ABIN6656050

Supplier Product No.: 200-301-a27

The Mouse Monoclonal anti-HSP70 1A antibody has been validated for WB, IHC, ELISA, FACS and FM. It is suitable to detect HSP70 1A in samples from Human. There are 3+ publications available.

Quick Overview for HSP70 1A antibody (AA 436-503) (ABIN6656050)

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: C92F3A-5

Product Details anti-HSP70 1A Antibody

Binding Specificity: AA 436-503

Supplier Product No.: 200-301-a27

Supplier: Rockland

Purpose: Hsp70 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.

Purification: This Protein G purified monoclonal antibody reacts with human and mouse Hsp70 protein.

Sterility: Sterile filtered

Immunogen: Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 10 μg/mL

IF_Microscopy_Dilution: 1:200-1:1000

Western_Blot_Dilution: 1:500-1:2000

Other: User Optimized

Comment:

Suggested Applications: Other
This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract.  In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 μg of heat shocked HeLa cell lysate by western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Hsp70 antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-HSP70 1A antibody (ABIN6656050)

Satoh, Stalder, McKercher, Williamson, Roth, Lipton: "Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential." in: ASN neuro, Vol. 7, Issue 4, (2016) (PubMed).

Ristic, Tsou, Guzi, Kanack, Scaglione, Todi: "USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila." in: The Journal of biological chemistry, Vol. 291, Issue 17, pp. 9161-72, (2016) (PubMed).

Satoh, Rezaie, Seki, Sunico, Tabuchi, Kitagawa, Yanagitai, Senzaki, Kosegawa, Taira, McKercher, Hoffman, Roth, Lipton: "Dual neuroprotective pathways of a pro-electrophilic compound via HSF-1-activated heat-shock proteins and Nrf2-activated phase 2 antioxidant response enzymes." in: Journal of neurochemistry, Vol. 119, Issue 3, pp. 569-78, (2011) (PubMed).

Target Details for HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Alternative Name: HSPA1A

Background:

Synonyms: Heat shock 70 kDa protein 1 antibody, heat shock 70 kDa protein 1A antibody, Heat shock 70 kDa protein 1B antibody, Heat shock induced protein antibody, heat shock protein 70 antibody, HSP70 1 antibody

Background: Anti-Hsp 70 antibody is routinely used in cancer and signal transduction research. Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

Gene Name: HSPA1A

Gene ID: 3304

NCBI Accession: NP_005336

UniProt: P0DMV8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process