HSPD1 antibody

Catalog No. ABIN6656053

Supplier Product No.: 200-401-f62

The Rabbit Polyclonal anti-HSPD1 antibody has been validated for WB, IHC, IF, ELISA and IP. It is suitable to detect HSPD1 in samples from Human. There is 1 publication available.

Quick Overview for HSPD1 antibody (ABIN6656053)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Product Details anti-HSPD1 Antibody

Supplier Product No.: 200-401-f62

Supplier: Rockland

Purpose: HSP60 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp60 from Human, Mouse, Rat, Bovine, Canine, Chicken, and Rabbit based on 100 % homology with the immunizing sequence.

Purification: Anti-HSP60 Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Hsp60 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against human Hsp60 produced through recombinant DNA methods in E.coli.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-HSP60 Antibody is tested for use in WB, IHC, IF and IP. Expect a band approximately ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-HSPD1 antibody (ABIN6656053)

Araújo de Lima, Oliveira Cunha, Felicio Calou, Tavares Neves, Facundo, Socorro de Barros Viana: "Effects of vitamin D (VD3) supplementation on the brain mitochondrial function of male rats, in the 6-OHDA-induced model of Parkinson's disease." in: Neurochemistry international, Vol. 154, pp. 105280, (2022) (PubMed).

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: Hsp60

Background:

Synonyms: CPN60, GROEL, HLD4, Hsp 60, Hsp65, HSPD1, HuCHA60, SPG 13, 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, Mitochondrial matrix protein P1, P60 lymphocyte protein, PD1

Background: In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60. Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes.

Gene Name: HSPD1

Gene ID: 3329

NCBI Accession: NP_002147

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity