SF3B4 antibody

Catalog No. ABIN6656066

Supplier Product No.: 200-301-x27

This anti-SF3B4 antibody is a Mouse Monoclonal antibody detecting SF3B4 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for SF3B4 antibody (ABIN6656066)

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SF3B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3A1

Product Details anti-SF3B4 Antibody

Supplier Product No.: 200-301-x27

Supplier: Rockland

Purpose: SAP 49 Antibody

Purification: Anti-SAP 49 Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-SAP 49 Antibody was produced in mice by repeated immunizations with a recombinant full length human SAP49 expressed in and purified from E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:2000

Other: ICC: 1:1000

Comment:

Anti-SAP 49 Antibody is tested for Western Blot, immunocytochemistry, immunohistochemistry, and is specific for the ~49kDa SAP49 protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for SF3B4

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Alternative Name: SAP 49

Background:

Synonyms: Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3B 49 kDa subunit, SF3b50, Spliceosome-associated protein 49

Background: The spliceosome associated protein of 49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145. The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex. SAP 49 Antibody is ideal for researchers interested in cell cycle research.

Gene Name: SF3B4

Gene ID: 10262

NCBI Accession: NP_005841

UniProt: Q15427