NeuN antibody (N-Term)

Catalog No. ABIN6656067

Supplier Product No.: 200-301-x09

This Mouse Monoclonal antibody specifically detects NeuN in WB, IHC, ELISA and FM. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for NeuN antibody (N-Term) (ABIN6656067)

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Clone: 1B7

Product Details anti-NeuN Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-301-x09

Supplier: Rockland

Purpose: FOX3 (NeuN) Antibody

Purification: Anti-FOX3 Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-FOX3 Antibody was produced in mice by repeated immunizations with a recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:200-1:1000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2000

Other: ICC: 1:200-1:1000

Comment:

Suggested Applications: Other
Anti-FOX3 Antibody is tested for Western Blot, immunohistochemistry, and immunocytochemistry, and it is specific for the ~46/48 kDa FOX3 protein doublet. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage upon thawing. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

References for anti-NeuN antibody (ABIN6656067)

Zhou, Wang, Zha: "RNA-Seq analysis of knocking out the neuroprotective proton-sensitive GPR68 on basal and acute ischemia-induced transcriptome changes and signaling in mouse brain." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 35, Issue 4, pp. e21461, (2021) (PubMed).

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: FOX3 (NeuN)

Background:

Synonyms: RNA binding protein fox-1 homolog 3, Fox-1 homolg C, NeuN, hexaribonucleotide binding protein 3

Background: FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing. FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development. FOX3 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: RBFOX3

Gene ID: 146713

NCBI Accession: NP_001076044

UniProt: A6NFN3