GRP78 antibody

Catalog No. ABIN6656070

Supplier Product No.: 200-301-f36

This Mouse Monoclonal antibody specifically detects GRP78 in WB, IF and FM. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for GRP78 antibody (ABIN6656070)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 1H11-1H7

Product Details anti-GRP78 Antibody

Supplier Product No.: 200-301-f36

Supplier: Rockland

Purpose: GRP78 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-GRP78 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.

Isotype: IgG2b

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-GRP78 Antibody has been tested by western blot and immunocytochemistry/immunofluorescence. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GRP78 antibody (ABIN6656070)

Hong, Tseng, Wala, Kim, Kynnap, Doshi, Kugener, Sandoval, Howard, Li, Yang, Tillgren, Ghandi, Sayeed, Deasy, Ward, McSteen, Labella, Keskula, Tracy, Connor, Clinton, Church, Crompton, Janeway et al.: "Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition. ..." in: eLife, Vol. 8, (2019) (PubMed).

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: GRP78

Background:

Synonyms: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Background: GRP78 is a ubiquitously expressed, 78- kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer's patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs.

Gene Name: Hspa5

Gene ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling