Choline Acetyltransferase antibody

Catalog No. ABIN6656077

Supplier Product No.: 600-101-d16

The Goat Polyclonal anti-Choline Acetyltransferase antibody has been validated for WB and IHC. It is suitable to detect Choline Acetyltransferase in samples from Human. There is 1 publication available.

Quick Overview for Choline Acetyltransferase antibody (ABIN6656077)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Choline Acetyltransferase Antibody

Supplier Product No.: 600-101-d16

Supplier: Rockland

Purpose: ChAT (Choline Acetyltransferase) Antibody

Cross-Reactivity (Details): Anti-ChAT antibody is directed against human ChAT protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:100

Comment:

Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 5 mg BSA

Preservative: 0.02 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Choline Acetyltransferase antibody (ABIN6656077)

Liu, Kim, Kim, Zhang, Bao, Denaxa, Lim, Kim, Liu, Wickersham, Pachnis, Hattar, Song, Brown, Blackshaw: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT (Choline Acetyltransferase)

Background:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development