SMAD7 antibody

Catalog No. ABIN6656373

Supplier Product No.: 200-401-i16

This Rabbit Polyclonal antibody specifically detects SMAD7 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SMAD7 antibody (ABIN6656373)

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SMAD7 Antibody

Supplier Product No.: 200-401-i16

Supplier: Rockland

Purpose: SMAD7 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-SMAD7 from human, rat, sheep and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-SMAD7 Antibody was purified by Protein G chromatography.

Immunogen: SMAD7 Antibody was produced from whole rabbit serum prepared by repeated immunizations with SMAD7 peptide.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 2-5 μg/mL

Comment:

Anti-SMAD7 antibody is tested for use in WB, ELISA, and IHC. Expect a band approximately 46kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: SMAD7

Background:

Synonyms: MADH7, MADH8

Background: Anti-SMAD7 MADH7 antibody detects human SMAD7 MADH7. SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Anti-SMAD7 MADH7 antibody is ideal for investigators involved kinase and phosphatase research.

Gene Name: SMAD7

Gene ID: 4092

NCBI Accession: NP_001177750

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization