TAO Kinase 2 antibody (pSer181)

Catalog No. ABIN6656508

Product Details anti-TAO Kinase 2 Antibody

Target: TAO Kinase 2 (TAOK2)

Binding Specificity: pSer181

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAO Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-TAO2 pS181 Antibody is directed against human TAO2 protein phosphorylated at Ser181. TAO2 pS181 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. Reactivity is expected from the following species based on 100% sequence homology: zebra fish, xenopus, finch and canine.

Immunogen:

Immunogen: Anti-TAO2 pS181 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 of TAO2 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-TAO2 pS181 (Rabbit) antibody is suitable for use in Western Blotting. Expect a band of approximately 120 kDa in size corresponding to TAO2 protein phosphorylated at Ser181 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for TAO Kinase 2

Target: TAO Kinase 2 (TAOK2)

Alternative Name: TAO2 (TAOK2 Products)

Synonyms: MAP3K17 antibody, PSK antibody, PSK1 antibody, PSK1-BETA antibody, TAO1 antibody, TAO2 antibody, Tao2 antibody, 1110033K02Rik antibody, B230344N16 antibody, mKIAA0881 antibody, TAOK2 antibody, map3k17 antibody, psk antibody, psk1 antibody, tao1 antibody, tao2 antibody, TAO kinase 2 antibody, TAO kinase 2 L homeolog antibody, serine/threonine-protein kinase TAO2 antibody, TAOK2 antibody, Taok2 antibody, taok2.L antibody, taok2 antibody, LOC100085396 antibody

Background:

Synonyms: Serine/threonine-protein kinase TAO2, Kinase from chicken homolog C, hKFC-C, Prostate-derived sterile 20-like kinase 1, PSK-1, Thousand and one amino acid protein kinase 2

Background: TAO2pS181 Antibody detects TAO2. In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK. This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs. Autophosphorylation of TAO2 may play a role in the mechanism of TAO2 activation. The MEK binding domain of TA2O is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain. Anti-TAO2 pS181 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: TAOK2

Gene ID: 9344

UniProt: Q9UL54

Pathways: Cell-Cell Junction Organization