MECP2 antibody (pSer80)

Catalog No. ABIN6656567

Supplier Product No.: 100-401-d71

This anti-MECP2 antibody is a Rabbit Polyclonal antibody detecting MECP2 in WB and IHC. Suitable for Human.

Quick Overview for MECP2 antibody (pSer80) (ABIN6656567)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MECP2 Antibody

Binding Specificity: pSer80

Supplier Product No.: 100-401-d71

Supplier: Rockland

Purpose: MeCP2 Phospho S80 Antibody

Cross-Reactivity (Details): This antibody is specific for the ~75 kDa MECP2 protein phosphorylated at Ser 80 in Western blots of human, rat and mouse brain extracts.

Purification: Anti-MeCP2 pS80 antibody was prepared from monospecific neat serum.

Immunogen: Anti-MeCP2 pS80 Antibody was produced in rabbit by repeated immunizations with MeCP Ser80 - Synthetic peptide corresponding to amino acid residues surrounding Ser 80 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-MeCP2 pS80 (Rabbit) has been tested in Western Blotting and IHC. Expect ~75kDa in WB. Anti-MeCP2 pS80 antibodies have been used successfully for IHS on mouse brain sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background:

Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein

Background: Anti-MeCP2 pS80 antibody detects MeCP2 when phosphorylated at Ser80. MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors.

Gene Name: MECP2

Gene ID: 4204

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane