TLR9 antibody

Catalog No. ABIN6656659

Supplier Product No.: 200-301-i31

The Mouse Monoclonal anti-TLR9 antibody has been validated for WB, IHC, FACS, IP, IF and DB. It is suitable to detect TLR9 in samples from Human.

Quick Overview for TLR9 antibody (ABIN6656659)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB)

Clone: 26C593-2

Product Details anti-TLR9 Antibody

Supplier Product No.: 200-301-i31

Supplier: Rockland

Purpose: TLR9 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR9 from monkey based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR9 Antibody was purified by Protein G chromatography.

Immunogen: TLR9 Antibody was produced in mice prepared repeated immunizations with amino acids corresponding to an internal sequence of human TLR9 isoform A protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 10-20 μg/mL (frozen)

Flow_Cytometry_Dilution: 0.1-2 μg/10^6 cells

Western_Blot_Dilution: 1-5 μg/mL

Comment:

Anti-TLR9 Antibody is tested for use in WB, DB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 113kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9

Background:

Synonyms: Toll-like receptor 9

Background: Anti-TLR9 CD289 Antibody detects human TLR9 CD289. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, a cDNA coding for TLR9 has been identified and subsequently isolated. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5 % . TLR9 is highly expressed in spleen. Anti-TLR9 CD289 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR9

Gene ID: 54106

NCBI Accession: NP_059138

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades