Aquaporin 1 antibody (N-Term)

Catalog No. ABIN6656923

Product Details anti-Aquaporin 1 Antibody

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Aquaporin 1 Antibody was purified by affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Aquaporin 1 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Other species not yet tested. Cross-reactivity with Aquaporin 1 from other sources has not been determined. Channels and Transporters research.

Immunogen:

Immunogen: Aquaporin 1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the N-terminal region.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aquaporin 1 Antibody is suitable for use in WB and IF microscopy. Expect a band approximately ~28.5 kDa on specific lysates. May detect larger glycosylated bands ~35-50 kDa. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000-4000

IF Microscopy Dilution: 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin 1 (AQP1 Products)

Synonyms: fj33g04 antibody, zgc:85890 antibody, wu:fj33g04 antibody, AQP1 antibody, CHIP28 antibody, AQP-CHIP antibody, CO antibody, AQP-1 antibody, CHIP29 antibody, aquaporin 1a (Colton blood group), tandem duplicate 1 antibody, aquaporin 1 antibody, aquaporin 1 (Colton blood group) antibody, aqp1a.1 antibody, AQP1 antibody, Aqp1 antibody

Background:

Synonyms: AQP1, Water channel protein CHIP29, Aquaporin-CHIP, Water channel protein for red blood cells and kidney proximal tubule, Aquaporin-1, Chip28

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin.

Gene Name: Aqp1

Gene ID: 25240

NCBI Accession: NP_036910

UniProt: P29975

Pathways: Hormone Transport