AQP3 antibody (C-Term)

Catalog No. ABIN6656926

Supplier Product No.: 600-401-e91

The Rabbit Polyclonal anti-AQP3 antibody has been validated for WB and FM. It is suitable to detect AQP3 in samples from Rat. There is 1 publication available.

Quick Overview for AQP3 antibody (C-Term) (ABIN6656926)

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP3 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Product Details anti-AQP3 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-e91

Supplier: Rockland

Purpose: Aquaporin 3 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Aquaporin 3 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Aquaporin 3 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Aquaporin 3 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of rat aquaporin 3.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:200-400

Western_Blot_Dilution: 1:1000-4000

Comment:

Suggested Applications: IF
Anti-Aquaporin 3 Antibody is suitable for use in WB and IF microscopy. Expect a band approximately ~31.5kDa on specific lysates. May detect larger glycosylated bands ~35-50kDa. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-AQP3 antibody (ABIN6656926)

Posfai, Maher, Roesch, Vantaux, Sylvester, Péneau, Popovici, Kyle, Witkowski, Derbyshire: "Plasmodium vivax Liver and Blood Stages Recruit the Druggable Host Membrane Channel Aquaporin-3." in: Cell chemical biology, Vol. 27, Issue 6, pp. 719-727.e5, (2021) (PubMed).

Target Details for AQP3

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Alternative Name: Aquaporin 3

Background:

Synonyms: AQP3, Aquaporin 3 (GIL blood group), GIL, 31.4 kDa water channel protein, Aquaglyceroporin-3

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. AQP3 gene expression is not regulated by vasopressin.

Gene Name: Aqp3

Gene ID: 65133

NCBI Accession: NP_113891

UniProt: P47862