COBRA1 antibody

Catalog No. ABIN6657338

Product Details anti-COBRA1 Antibody

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COBRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-COBRA 1 antibody was Protein G purified from culture supernatant. This antibody is directed against human COBRA 1. Reactivity is expected against mouse and rat sources. Cross-reactivity from other sources has not been determined.

Immunogen:

Immunogen: Anti-COBRA1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human COBRA1 purified from E.coli.

Immunogen Type: Recombinant Protein

Clone: F7E4

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-COBRA 1 antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 62 kDa in size corresponding to COBRA1 proteins in neonatal rat brain lysate or other appropriate lysates.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: COBRA 1 (COBRA1 Products)

Synonyms: LOC100219583 antibody, COBRA1 antibody, NELF-B antibody, bZ1O1.4 antibody, cobra1 antibody, cobra2 antibody, fe01e12 antibody, wu:fe01e12 antibody, zgc:55628 antibody, MGC131073 antibody, Cobra1 antibody, RGD1307832 antibody, A730008L03Rik antibody, AB041607 antibody, AI663983 antibody, Nelf-b antibody, cofactor of BRCA1 antibody, negative elongation factor complex member B antibody, negative elongation factor complex member B L homeolog antibody, LOC100219583 antibody, NELFB antibody, nelfb antibody, nelfb.L antibody, Nelfb antibody

Background:

Synonyms: COBRA1, Negative elongation factor B, NELF-B, Cofactor of BRCA1

Background: COBRA 1 antibody reacts with COBRA1 (cofactor of BRCA1) also known as NELF-B, which is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription. COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis. Anti-COBRA 1 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: COBRA1

Gene ID: 25920

UniProt: Q8WX92