ALOX5 antibody (pSer523)

Catalog No. ABIN6657368

Supplier Product No.: 600-401-d03

This anti-ALOX5 antibody is a Rabbit Polyclonal antibody detecting ALOX5 in WB and ELISA. Suitable for Human.

Quick Overview for ALOX5 antibody (pSer523) (ABIN6657368)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ALOX5 Antibody

Binding Specificity: pSer523

Supplier Product No.: 600-401-d03

Supplier: Rockland

Purpose: 5-Lipoxygenase phospho S523 Antibody

Cross-Reactivity (Details): Anti-5-Lipoxygenase pS523 Antibody is directed against human 5-Lipoxygenase phosphorylated at S523.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-5-Lipoxygenase pS523 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-5-Lipoxygenase pS523 antibody has been tested in Western Blotting and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 80 kDa corresponding to the 80 kDa 5-LO proteins phosphorylated at Ser523.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: 5-Lipoxygenase

Background:

Synonyms: Arachidonate 5-lipoxygenase, 5-LO

Background: 5-Lipoxygenase pS523 antibody recognizes the enzyme 5-lipoxygenase (5-LO) which plays a key role in regulating the production of leukotrienes (LTs). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma, fibrosis and atherosclerosis. Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO. Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. Anti-5-Lipoxygenase pS523 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: ALOX5

Gene ID: 240

UniProt: P09917