PCLO antibody

Catalog No. ABIN6657442

Supplier Product No.: 200-301-g30

The Mouse Monoclonal anti-PCLO antibody has been validated for WB, FM, ELISA and IF. It is suitable to detect PCLO in samples from Human.

Quick Overview for PCLO antibody (ABIN6657442)

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCLO antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM), ELISA, Immunofluorescence (IF)

Clone: 6H9-B6

Product Details anti-PCLO Antibody

Supplier Product No.: 200-301-g30

Supplier: Rockland

Purpose: Piccolo Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Piccolo from human, mouse, rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Piccolo Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Piccolo Antibody was produced in mice by repeated immunizations raised against a full length protein.

Isotype: IgG2b kappa

Application Details

Application Notes:

ELISA_Dilution: 1:200

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-Piccolo Antibody is tested for use in WB, ICC/IF, and ELISA. Expect a band ~550kDa in specific lysate or tissue samples. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PCLO

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Alternative Name: PCLO

Background:

Synonyms: ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody, protein piccolo, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein

Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

Gene Name: Pclo

Gene ID: 27445

NCBI Accession: NP_055325

UniProt: Q9Y6V0

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis