ELK1 antibody (pSer383)

Catalog No. ABIN6657457

Supplier Product No.: 600-401-d34

The Rabbit Polyclonal anti-ELK1 antibody has been validated for WB and ELISA. It is suitable to detect ELK1 in samples from Human.

Quick Overview for ELK1 antibody (pSer383) (ABIN6657457)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ELK1 Antibody

Binding Specificity: pSer383

Supplier Product No.: 600-401-d34

Supplier: Rockland

Purpose: Elk-1 phospho S383 Antibody

Cross-Reactivity (Details): Anti-Elk-1 pS383 antibody is directed against Elk-1.

Purification: Anti-Elk-1 pS383 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Elk-1 pS383 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Elk-1 pS383 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to Elk-1 phosphorylated at Ser383 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: Elk-1

Background:

Synonyms: ETS domain-containing protein Elk-1

Background: Elk-1 Antibody detects Elk-1 which is a transcription factor involved in mediating gene transcription in response to growth factors. Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1. Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus. Anti-Elk-1 pS383 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility and, Cancer Research.

Gene Name: ELK1

Gene ID: 2002

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling