Talin-1 (LOC102565709) antibody

Catalog No. ABIN6657478

Supplier Product No.: 200-301-p47

The Mouse Monoclonal anti-Talin-1 antibody is suitable to detect Talin-1 in samples from Chicken. It has been validated for ELISA and WB.

Quick Overview for Talin-1 (LOC102565709) antibody (ABIN6657478)

Target: Talin-1 (LOC102565709)

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Clone: 8d4

Product Details

Supplier Product No.: 200-301-p47

Supplier: Rockland

Purpose: Talin Antibody

Cross-Reactivity (Details): This product reacts with human and bovine.

Purification: Anti-Talin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Talin antibody was produced in mice by repeated immunizations with purified chicken gizzard talin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:40,000

Western_Blot_Dilution: 2-4 μg/mL

Comment:

Anti-Talin is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~270-272 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Talin-1 (LOC102565709)

Alternative Name: TLN1/TLN2

Background:

Synonyms: TLN, KIAA1027, ILWEQ, TALIN

Background: Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix (ECM) receptors. It has a mass of 270 kD and shares 23 % N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p. This antibody is suitable for researchers interested in cell signaling, membrane biology, and growth factor research.

Gene Name: TLN1, TLN2

Gene ID: 7094

NCBI Accession: NP_006280

UniProt: Q9Y490