NOS1 antibody (AA 1-181)

Catalog No. ABIN6657480

Supplier Product No.: 200-301-p43

This Mouse Monoclonal antibody specifically detects NOS1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NOS1 antibody (AA 1-181) (ABIN6657480)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: NOS-B1

Product Details anti-NOS1 Antibody

Binding Specificity: AA 1-181

Supplier Product No.: 200-301-p43

Supplier: Rockland

Purpose: Nitric Oxide Synthase (NOS1) Antibody

Cross-Reactivity (Details): This product reacts with human, rat, goat, and pig.

Purification: Anti-NOS1 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Nitric Oxide Synthase (NOS1) antibody was produced in mice by repeated immunizations with recombinant protein corresponding to neuronal NOS fragment (amino acids 1-181) from rat brain.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:5000

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-NOS1 is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~161 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: Nitric Oxide Synthase (NOS1)

Background:

Synonyms: NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1

Background: Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior. This antibody is suitable for researchers interested in apoptosis research, cell death, and TNF pathways.

Gene Name: NOS1

Gene ID: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction