Cyclin D2 antibody

Catalog No. ABIN6657481

Supplier Product No.: 200-301-p32

The Mouse Monoclonal anti-Cyclin D2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Cyclin D2 in samples from Human.

Quick Overview for Cyclin D2 antibody (ABIN6657481)

Target: Cyclin D2 (CCND2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: DCS-3

Product Details anti-Cyclin D2 Antibody

Supplier Product No.: 200-301-p32

Supplier: Rockland

Purpose: Cyclin D2 Antibody

Cross-Reactivity (Details): This product reacts with human and mouse.

Purification: Anti-Cyclin D2 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Cyclin D2 antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to human cyclin D2 protein.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:40,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 2-4 μg/mL

Other: Immunocytochemistry: 1 μg/mL

Comment:

Anti-Cyclin D2 is tested for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~33 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cyclin D2

Target: Cyclin D2 (CCND2)

Alternative Name: Cyclin D2

Background:

Synonyms: KIAK0002

Background: Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13. This antibody is suitable for researchers interested in cancer research, autoimmune disease, neurodegenerative disease, MAP Kinase Signaling, AKT Signaling, Ubiquitin research, and Cell Cycle research.

Gene Name: CCND2

Gene ID: 894

NCBI Accession: NP_001750

UniProt: P30279

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases