Nitrotyrosine antibody

Catalog No. ABIN6657486

Supplier Product No.: 200-301-a98

This Mouse Monoclonal antibody specifically detects Nitrotyrosine in WB, ELISA and IHC. It exhibits reactivity toward .

Quick Overview for Nitrotyrosine antibody (ABIN6657486)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 39B6

Product Details anti-Nitrotyrosine Antibody

Supplier Product No.: 200-301-a98

Supplier: Rockland

Purpose: Nitrotyrosine Antibody

Cross-Reactivity (Details): This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources.

Purification: This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues.

Sterility: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 0.7 μg of antibody is sufficient to detect 5 μg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background:

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Gene Name: n/a