Desmin antibody

Catalog No. ABIN6657489

Supplier Product No.: 200-301-p35

This Mouse Monoclonal antibody specifically detects Desmin in WB, IHC and ELISA. It exhibits reactivity toward Pig.

Quick Overview for Desmin antibody (ABIN6657489)

Target: Desmin (DES)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: DE-U-10

Product Details anti-Desmin Antibody

Supplier Product No.: 200-301-p35

Supplier: Rockland

Purpose: Desmin Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, rat, and rabbit.

Purification: Anti-Desmin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Desmin antibody was produced in mice by repeated immunizations with desmin from pig stomach.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 2 μg/mL

Comment:

Anti-Desmin is tested for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~53.5 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background:

Synonyms: CMD1I, CSM1, CSM2, intermediate filament protein

Background: Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, neurodegenerative diseases, cell adhesion research, and cytoskeletal signaling research.

Gene Name: DES

Gene ID: 396725

NCBI Accession: NP_001001535

UniProt: P02540