Apelin antibody (C-Term)

Catalog No. ABIN6657507

Product Details anti-Apelin Antibody

Target: Apelin (APLN)

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apelin antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Apelin antibody is directed against human Apelin protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence.  Cross reactivity with Apelin from other sources has not been determined.

Immunogen:

Immunogen: Apelin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Apelin.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Apelin is useful for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~8.6 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Apelin

Target: Apelin (APLN)

Alternative Name: Apelin (APLN Products)

Synonyms: APLN antibody, Xapelin antibody, xnpep2 antibody, preproapelin antibody, 6030430G11Rik antibody, Apel antibody, APEL antibody, XNPEP2 antibody, apelin antibody, apelin S homeolog antibody, apln antibody, APLN antibody, apln.S antibody, Apln antibody

Background:

Synonyms: APJ endogenous ligand,

Background: Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The apelin gene to Xq25-q26.3 based on sequence similarity between the apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. This antibody is suitable for researchers interested in neuroscience research.

Gene Name: APLN

Gene ID: 8862

NCBI Accession: NP_059109

UniProt: Q9ULZ1

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour