ITGA3 antibody (N-Term)

Catalog No. ABIN6657511

Supplier Product No.: 600-401-r13

The Rabbit Polyclonal anti-ITGA3 antibody has been validated for WB, FACS, IHC and FM. It is suitable to detect ITGA3 in samples from Human.

Quick Overview for ITGA3 antibody (N-Term) (ABIN6657511)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-ITGA3 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-r13

Supplier: Rockland

Purpose: ITGA3 Antibody

Cross-Reactivity (Details): Anti-ITGA3 antibody is directed against human ITGA3 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: ITGA3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of human ITGA3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 2-5 μg/mL

Flow_Cytometry_Dilution: 1-3 μg/1x10^6 cells

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Comment:

Anti-ITGA3 is tested for Immunohistochemistry -P, ICC/IF, Flow Cytometry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~116.6 kDa corresponding to the appropriate cell lysate or extract. 0.05 mg sodium azide and 0.05 mg Thimerosal have been added as preservatives.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: ITGA3

Background:

Synonyms: CD49 antigen-like family member C,FRP-2,Galactoprotein B3,

Background: ITGA3(INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. ITGA3 is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. ITGA3 is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the alpha3beta1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit.A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis. This antibody is suitable for researchers interested in cancer research.

Gene Name: ITGA3

Gene ID: 3675

NCBI Accession: NP_002195

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex