COL4a2 antibody (C-Term)

Catalog No. ABIN6657525

Supplier Product No.: 600-401-r11

This Rabbit Polyclonal antibody specifically detects COL4a2 in WB, IHC, IF and FM. It exhibits reactivity toward Human.

Quick Overview for COL4a2 antibody (C-Term) (ABIN6657525)

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-COL4a2 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-r11

Supplier: Rockland

Purpose: Canstatin Antibody

Cross-Reactivity (Details): Anti-Canstatin antibody is directed against human Canstatin protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Canstatin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Canstatin.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

IF_Microscopy_Dilution: 2 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: ICC: 2 μg/mL

Comment:

Anti-Canstatin is tested for Immunofluorescence, Immunohistochemistry -P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~168.6 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for COL4a2

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Alternative Name: Canstatin

Background:

Synonyms: Collagen alpha-2(IV) chain

Background: Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. This antibody is suitable for researchers interested in cancer and stem cell research.

Gene Name: COL4A2

Gene ID: 1284

NCBI Accession: NP_001837

UniProt: P08572