ZIC1 antibody (N-Term)

Catalog No. ABIN6657599

Product Details anti-ZIC1 Antibody

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZIC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine)

Purification: This is an IgG preparation of whole rabbit antiserum purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is directed against Zic-1 from mouse.  In general, this antibody also detects human Zic-1.  Cross-reactivity with other species is likely but has not been determined.

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with an 18 aa synthetic peptide from a region near the N-Terminus of mouse Zic-1. This domain is completely conserved in human ZIC-1.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:400

Application Note: Anti Zic-1 Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:5,000

IF Microscopy Dilution: 1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-ZIC1 antibody (ABIN6657599)

He, Yu, Lu, Wang, Wu, Zhao, Li, Zhou, Liu, Mu, Xin, Qiu, Lu: "Transcriptional Regulator ZEB2 Is Essential for Bergmann Glia Development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 6, pp. 1575-1587, (2019) (PubMed).

Sankar, Yellajoshyula, Zhang, Teets, Rockweiler, Kroll: "Gene regulatory networks in neural cell fate acquisition from genome-wide chromatin association of Geminin and Zic1." in: Scientific reports, Vol. 6, pp. 37412, (2018) (PubMed).

He, Zhang, Chen, Remke, Shih, Lu, Wang, Deng, Yu, Xia, Wu, Ramaswamy, Hu, Wang, Zhou, Burns, Kim, Kool, Pfister, Weinstein, Pomeroy, Gilbertson, Rubin, Hou, Wechsler-Reya, Taylor, Lu: "The G protein α subunit Gαs is a tumor suppressor in Sonic hedgehog-driven medulloblastoma." in: Nature medicine, Vol. 20, Issue 9, pp. 1035-42, (2014) (PubMed).

Zhao, Ayrault, Zindy, Kim, Roussel: "Post-transcriptional down-regulation of Atoh1/Math1 by bone morphogenic proteins suppresses medulloblastoma development." in: Genes & development, Vol. 22, Issue 6, pp. 722-7, (2008) (PubMed).

Target Details for ZIC1

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Alternative Name: Zic-1 (ZIC1 Products)

Synonyms: ZIC antibody, ZNF201 antibody, opl antibody, Zic family member 1 antibody, Zic family member 1 S homeolog antibody, zinc finger protein of the cerebellum 1 antibody, ZIC1 antibody, Zic1 antibody, zic1.S antibody

Background:

Synonyms: rabbit anti-Zic-1 Antibody, Odd paired homolog Drosophila antibody, Zic 1 antibody, ZIC antibody, Zic family member 1 (odd-paired Drosophila homolog) antibody, Zic family member 1 antibody

Background: Anti Zic-1 Antibody recognizes the product of the Zic-1 gene, that encodes a zinc finger protein which is expressed in the developing or matured central nervous system in a highly restricted manner.  Zic-1 is expressed in granule cells that make synaptic contact with Purkinje cells.  Clearly Zic-1 is a gene critical to cerebellar pattern formation.  The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm.  Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family.  By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation.  The Zic-1 gene has been mapped to chromosome 9 in mouse.  The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis.  The temporal profile of mRNA expression differs for each of the Zic gene products.  The Drosophila odd-paired gene is highly homologous to the Zic gene family.

Gene Name: Zic1

Gene ID: 22771

UniProt: P46684