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PIK3CD antibody (C-Term)

PIK3CD Reactivity: Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6657601
  • Target See all PIK3CD Antibodies
    PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))
    Binding Specificity
    • 23
    • 15
    • 11
    • 10
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 109
    • 42
    • 9
    Mouse
    Host
    • 103
    • 6
    Rabbit
    Clonality
    • 95
    • 14
    Polyclonal
    Conjugate
    • 37
    • 10
    • 9
    • 9
    • 7
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PIK3CD antibody is un-conjugated
    Application
    • 78
    • 58
    • 40
    • 26
    • 26
    • 18
    • 14
    • 8
    • 7
    • 7
    • 4
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity
    Human, Mouse (Murine)
    Cross-Reactivity (Details)
    Cross reactivity is expected against the human protein as the sequence of the immunogen is 100 % identical in both human and mouseCross reactivity is also expected against rat PI3K p110d.
    Purification
    This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.  Reactivity occurs against mouse PI3K p110d subunit. Cross reactivity is expected against the human protein as the sequence of the immunogen is 100% identical in both human and mouse. Cross reactivity is also expected against rat PI3K p110d.
    Immunogen

    Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of mouse PI3K p110d. This sequence is identical in both mouse and human.

    Immunogen Type: Peptide

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  • Application Notes

    Application Note: This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A mouse whole cell splenic lysate is suitable for use as a positive control.

    ELISA Dilution: 1:4,000 - 1:20,000

    Western Blot Dilution: 1:5,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Jou, Carpino, Takahashi, Piekorz, Chao, Carpino, Wang, Ihle: "Essential, nonredundant role for the phosphoinositide 3-kinase p110delta in signaling by the B-cell receptor complex." in: Molecular and cellular biology, Vol. 22, Issue 24, pp. 8580-91, (2003) (PubMed).

  • Target
    PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))
    Alternative Name
    PI3-kinase p110 delta (PIK3CD Products)
    Synonyms
    2410099E07Rik antibody, 2610208K16Rik antibody, AW545373 antibody, p110delta antibody, P110DELTA antibody, PI3K antibody, p110D antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta antibody, Pik3cd antibody, PIK3CD antibody
    Background

    Synonyms: rabbit anti-PI3-kinase p110 delta Antibody, p110D antibody, p110delta antibody, Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody, PI3K antibody, PIK3CD antibody

    Background: Phosphoinositide 3-kinases (PI3Ks) generate 3-phosphoinositide lipids in cell membranes.  A variety of intracellular target proteins interact with these lipids via specific lipid-binding modules and, as a consequence, undergo changes in their localization and/or activity. In this way, PI3Ks participate in the regulation of mitogenesis, differentiation, survival, intracellular vesicular transport, cytoskeletal reorganization, and motility.  Tyrosine kinases and Ras use PI3Ks as essential intracellular signal relay molecules. PI3Ks are heterodimeric enzymes consisting of a regulatory subunit in complex with a p110 catalytic subunit. Mammmals have genes encoding three distinct catalytic subunits (p110α, p110ß, and p110δ and three regulatory subunits (p85α, p85ß, and p55δ). All of the p110 isoforms are capable of interacting with each type of regulatory subunit. They are also similarly recruited to phosphotyrosine complexes and have, at least in vitro, the same lipid substrate specificity. However, it is becoming increasingly clear that PI3K isoforms differ in their interaction with Ras and regulation of lipid kinase activity, and in their protein kinase activities. Several groups have provided evidence that p110 isoforms have nonredundant functions in the regulation of cell proliferation, survival, actin cytoskeleton reorganization, and migration downstream of given receptors. This antibody is specific for the carboxy teminal end of p110d that is expressed predominantly in leukocytes.

    Gene Name: PIK3CD

    Gene ID
    18707
    UniProt
    O35904
    Pathways
    BCR Signaling, Warburg Effect
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