HSF1 antibody (AA 378-395) (HRP)

Catalog No. ABIN6657641

Supplier Product No.: 200-503-w88

This anti-HSF1 antibody is a Rat Monoclonal antibody detecting HSF1 in WB, ELISA, IP and GS. Suitable for Mouse.

Quick Overview for HSF1 antibody (AA 378-395) (HRP) (ABIN6657641)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Gel Shift (GS)

Clone: 10H4

Product Details anti-HSF1 Antibody (HRP)

Binding Specificity: AA 378-395

Supplier Product No.: 200-503-w88

Supplier: Rockland

Purpose: HSF1 Antibody Peroxidase

Purification: Anti-HSF1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378-395.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:10,000

Gel_Shift_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-HSF1 HRP Conjugated Antibody is suitable for Western Blots, Immunoprecipitation, ELISA, Gel Mobility Shift Assay and Immunocytochemistry. Expect a band approximately ~85kDa protein in unstressed cell lysates, and a 95 kDa protein in heat shocked cell lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Synonyms: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). Anti-HSF1 Antibody is ideal for research in Genetics, Transcription, Cell Signaling and pathways including ERK and MAPK.

Gene Name: Hsf1

Gene ID: 15499

NCBI Accession: NP_058761

UniProt: P38532