Ly6g antibody (FITC)

Catalog No. ABIN6657770

Supplier Product No.: 200-502-l54

Cited in 1 publication. The FITC-conjugated Rat Monoclonal anti-Ly6g antibody (Clone RB6-8C5) (ABIN6657770) specifically detects Ly6g in FACS, WB, IHC, IP and MA. The antibody is reactive with Mouse samples.

Quick Overview for Ly6g antibody (FITC) (ABIN6657770)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Multiplex Assay (MA)

Clone: RB6-8C5

Product Details anti-Ly6g Antibody (FITC)

Supplier Product No.: 200-502-l54

Supplier: Rockland

Purpose: Ly-6G/Ly-6C (Gr-1) Fluorescein Antibody

Cross-Reactivity (Details): Cross reactivity with Ly-6G/Ly-6C (Gr-1) from other sources has not been tested.

Purification: Fluorescein conjugated Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G/Ly-6C (Gr-1).

Sterility: Sterile filtered

Immunogen: Anti-Ly-6G/Ly-6C (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with raised against granulocytes of mouse origin.

Isotype: IgG2b kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Western_Blot_Dilution: User Optimized

Comment:

Anti-Ly-6G/Ly-6C (Gr-1) is tested for FLOW and useful for Immunohistochemistry, Immunoprecipitation, and Western Blot using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

References for anti-Ly6g antibody (FITC) (ABIN6657770)

Niu, Qu, Yang, Zhang, Yuan, Fan, Li, Tao: "Protective effect of HDACIs in improves survival and organ injury after CLP-induced sepsis." in: Surgery open science, Vol. 12, pp. 35-42, (2023) (PubMed).

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Gr-1

Background:

Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Background: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Gene Name: Gr-1

Gene ID: 17067

NCBI Accession: NP_001238984