Ly6g antibody (PE)

Catalog No. ABIN6657771

Supplier Product No.: 200-508-l54

This Rat Monoclonal antibody specifically detects Ly6g in FACS, WB, IHC and IP. It exhibits reactivity toward Mouse.

Quick Overview for Ly6g antibody (PE) (ABIN6657771)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is conjugated to PE

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: RB6-8C5

Product Details anti-Ly6g Antibody (PE)

Supplier Product No.: 200-508-l54

Supplier: Rockland

Purpose: Ly-6G/Ly-6C (Gr-1) Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with Ly-6G/Ly-6C (Gr-1) from other sources has not been tested.

Purification: Phycoerythrin conjugated Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G/Ly-6C (Gr-1).

Sterility: Sterile filtered

Immunogen: Anti-Ly-6G/Ly-6C (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with raised against granulocytes of mouse origin.

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Western_Blot_Dilution: User Optimized

Comment:

Anti-Ly-6G/Ly-6C (Gr-1) is tested in FLOW and useful for Immunohistochemistry and Immunoprecipitation, Western Blot using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Gr-1

Background:

Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Background: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Gene Name: Gr-1

Gene ID: 17067

NCBI Accession: NP_001238984