CD40 Ligand antibody (PE)

Catalog No. ABIN6657774

Supplier Product No.: 200-b08-n91

This Hamster Monoclonal antibody specifically detects CD40 Ligand in FACS and IHC. It exhibits reactivity toward Mouse.

Quick Overview for CD40 Ligand antibody (PE) (ABIN6657774)

Target: CD40 Ligand (CD40LG)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: MR1

Product Details anti-CD40 Ligand Antibody (PE)

Supplier Product No.: 200-b08-n91

Supplier: Rockland

Purpose: CD154 (CD40L) Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with CD154 from other sources has not been tested.

Purification: Phycoerythrin conjugated CD154 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD154.

Sterility: Sterile filtered

Immunogen: Anti-CD154 Antibody (Monoclonal) was produced by repeated immunizations with Activated mouse Th1 clone D1.6.

Isotype: IgG

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD154 is tested for FLOW and useful for Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154(CD0L)

Background:

Synonyms: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, Cd40lg, Cd40l, Tnfsf5

Background: CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.

Gene Name: Cd40lg

Gene ID: 21947

NCBI Accession: NP_035746

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints