Ly6g antibody (APC)

Catalog No. ABIN6657868

Supplier Product No.: 200-526-l54

The Rat Monoclonal anti-Ly6g antibody is suitable to detect Ly6g in samples from Mouse. It has been validated for FACS, IHC and IP.

Quick Overview for Ly6g antibody (APC) (ABIN6657868)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: RB6-8C5

Product Details anti-Ly6g Antibody (APC)

Supplier Product No.: 200-526-l54

Supplier: Rockland

Purpose: Ly-6G (Gr-1) Allophycocyanin Antibody

Cross-Reactivity (Details): Cross reactivity with Ly-6G (Gr-1) from other sources has not been tested.

Purification: Allophycocyanin conjugated Ly-6G (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G (Gr-1).

Sterility: Sterile filtered

Immunogen: Anti-Ly-6G (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with Ly-6G/Ly-6C (Gr-1) antigen.

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-Ly-6G (Gr-1) is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Ly-6G (Gr-1)

Background:

Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Background: The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

Gene Name: Gr-1

Gene ID: 17067

NCBI Accession: NP_001238984