NK-1.1/CD161c antibody (FITC)

Catalog No. ABIN6657870

Supplier Product No.: 200-302-n93

This Mouse Monoclonal antibody specifically detects NK-1.1/CD161c in FACS, IP, IHC and FM. It exhibits reactivity toward Mouse.

Quick Overview for NK-1.1/CD161c antibody (FITC) (ABIN6657870)

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: PK136

Product Details anti-NK-1.1/CD161c Antibody (FITC)

Supplier Product No.: 200-302-n93

Supplier: Rockland

Purpose: NK1.1 Fluorescein Antibody

Purification: Fluorescein conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.

Sterility: Sterile filtered

Immunogen: Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.

Isotype: IgG2a kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Other: Sufficient to run approximately 500 tests

Comment:

Anti-NK1.1 has been tested by Flow Cytometry and is useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Alternative Name: NK1.1

Background:

Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

Background: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

Gene Name: Klrb1c

Gene ID: 17059

NCBI Accession: NP_001153376

UniProt: P27814