NK-1.1/CD161c antibody (PE)

Catalog No. ABIN6657871

Supplier Product No.: 200-308-n93

The Mouse Monoclonal anti-NK-1.1/CD161c antibody has been validated for FACS, IP and IHC. It is suitable to detect NK-1.1/CD161c in samples from Mouse.

Quick Overview for NK-1.1/CD161c antibody (PE) (ABIN6657871)

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: PK136

Product Details anti-NK-1.1/CD161c Antibody (PE)

Supplier Product No.: 200-308-n93

Supplier: Rockland

Purpose: NK1.1 Phycoerythrin Antibody

Purification: Phycoerythrin conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.

Sterility: Sterile filtered

Immunogen: Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK-1+ cells from mouse spleen and bone marrow.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-NK1.1 tested in Flow Cytometry and is useful for Immunohistochemistry, Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Alternative Name: NK1.1

Background:

Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

Background: NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Gene Name: Klrb1c

Gene ID: 17059

NCBI Accession: NP_001153376

UniProt: P27814