CD19 antibody (PE)

Catalog No. ABIN6657889

Supplier Product No.: 200-508-n81

The Rat Monoclonal anti-CD19 antibody is suitable to detect CD19 in samples from Mouse. It has been validated for FACS and IP.

Quick Overview for CD19 antibody (PE) (ABIN6657889)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 6D5

Product Details anti-CD19 Antibody (PE)

Supplier Product No.: 200-508-n81

Supplier: Rockland

Purpose: CD19 Phycoerythrin Antibody

Cross-Reactivity (Details): Cross-reactivity with CD19 from other sources has not been tested.

Purification: Phycoerythrin conjugated CD19 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD19.

Sterility: Sterile filtered

Immunogen: Anti-CD19 Antibody (Monoclonal) was produced by repeated immunizations with Mouse CD19-expressing K562 human erythroleukemia cells.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD19 is tested for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background:

Synonyms: B-lymphocyte antigen CD19, Differentiation antigen CD19, CD19, Cd19

Background: CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Gene Name: Cd19

Gene ID: 12478

NCBI Accession: NP_033974

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway